The major research efforts of our laboratory are aimed at the identification, molecular characterization and understanding of host-pathogen interactions with respect to novel virulence factors of multidrug resistant and fastidious bacteria and viruses. We focus on virulence factors and biochemical pathways needed for pathogenicity that can be targeted using novel therapeutic approaches that lower the risks of developing drug resistance. Specifically, we are interested in those molecular targets with a general effect on the infection process, like those affecting attachment, biofilm formation or which simultaneously regulate multiple virulence mechanisms. We are using the opportunistic pathogen Pseudomonas aeruginosa as a model because of its versatility of virulence strategies for different hosts, which provides different models in which to examine bacterial factors important for the pathogenicity process. P. aeruginosa’s extensively studied biofilm also provides an excellent model to study factors affecting the physiology of the biofilm, which is essential for persistence and resistance to antibiotics and host defenses in the context of important diseases such as cystic fibrosis and chronic obstructive pulmonary disease.